human lung cancer epithelial cells a549  (ATCC)

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Ginkgolide a enhances the resistance to pathogen infection through mitochondrial unfolded protein response

doi: 10.1007/s00018-025-05869-5

Figure Lengend Snippet: GA protects lung epithelial cells from infection through activation of SATB2 mediated UPR mt ( A ) The cytotoxicity of cells exposed to P. aeruginosa PA14 for 4 h was significantly reduced in A549 lung epithelial cells after GA treatment. (** P < 0.01, unpaired t-test). ( B ) The mRNA level of SATB2, after GA treatment. These results are mean ± SEM of three independent experiments performed in triplicate. (** P < 0.01, unpaired t-test). ( C ) Quantitative real-time PCR was used to measure the knockdown efficiency of SATB2. (** P < 0.01, unpaired t-test). ( D ) The cytotoxicity of cells exposed to P. aeruginosa PA14 for 4 h in SATB2 knockdown and Control A549 lung epithelial cells after GA treatment. (** P < 0.01, ANOVA). ( E ) The mRNA levels of the mitochondrial unfolded protein response genes in SATB2 knockdown and Control A549 lung epithelial cells after GA treatment. These results are mean ± SEM of three independent experiments performed in triplicate (* P < 0.05, unpaired t-test)

Article Snippet: A549 human lung cancer epithelial cells (American Type Culture Collection, Manassas, VA, USA) were rinsed and incubated in a serum-free medium for 1 h. Subsequently, the cells were infected with P. aeruginosa PA14, prepared in PBS from the logarithmic growth phase, at a multiplicity of infection of 100:1 [ ].

Techniques: Infection, Activation Assay, Real-time Polymerase Chain Reaction, Knockdown, Control